BPC-157 · 5mg lyophilized vial · reconstitution · research use only
BPC-157 reconstitution: the water volume is the only variable that sets your concentration.
Titan's BPC-157 ships as a 5mg lyophilized powder in a single vial. The powder mass is fixed — what you decide is how much bacteriostatic water to add, and that one choice sets the concentration for every aliquot you draw afterwards. BPC-157 is a microgram-scale compound, so on a standard U-100 insulin syringe the draws are small and the arithmetic matters. This page works through the exact mg/mL and microgram-per-unit math for the common 1mL, 2mL and 3mL volumes, plus how to add the water without shearing the peptide and how to read identity off a lot-matched COA. It is a laboratory reference, not a human protocol or dosing advice.
5mg is fixed — the water sets everything
The vial holds 5mg of BPC-157 regardless of how it's reconstituted. The volume of bacteriostatic water you add is what determines concentration: more water means a more dilute, easier-to-measure solution; less water means a concentrated one where each unit on the syringe carries more peptide. Pick the volume first, then the per-unit math falls out of it — the powder mass never changes.
Which diluent to use →The three common volumes, worked out
Add 1mL and 5mg dissolves into 5mg/mL (5000mcg/mL) — on a U-100 syringe that's 50mcg per unit. Add 2mL and you get 2.5mg/mL (2500mcg/mL), or 25mcg per unit. Add 3mL and it's about 1.67mg/mL (~1667mcg/mL), roughly 16.7mcg per unit. Same 5mg, three concentrations, three different per-unit values — the spec table below lays them side by side.
BPC-157 research ranges →Reading a U-100 syringe
On a U-100 insulin syringe, 100 units equals 1mL, so one unit is 0.01mL. To pull a 250mcg research aliquot from a 2mL reconstitution (25mcg/unit), you draw 10 units — 0.1mL. The unit marks measure volume, not peptide; the concentration you set during reconstitution is what converts those marks into micrograms. Get the volume math right once and every later draw is just reading the line.
Run your own numbers →Add water gently, then store cold
Run the bacteriostatic water slowly down the inside of the vial wall rather than firing it onto the powder — peptides are sheared by aggressive mixing. Let it dissolve on its own or swirl gently; do not shake. Once reconstituted, BPC-157 is handled as a refrigerated solution with a finite working window, where the lyophilized powder was stable far longer. Plan the volume around how much you'll actually use before that window closes.
Storage & shelf-life notes →Concentration is only as good as identity
Accurate mg/mL math assumes the vial actually holds 5mg of correctly identified BPC-157. That is a COA question, not a calculator question: a lot-matched release sheet should confirm the pentadecapeptide sequence by mass spec and purity by HPLC, against the lot number on the vial in front of you. Titan's BPC-157 ships against an HPLC main-peak result with mass-spec identity confirmation.
How to verify a COA →Research-use framing
BPC-157 is supplied strictly as a research-use-only reagent. The reconstitution arithmetic here describes how to prepare a known concentration for in-vitro and modelling work — it is dilution math, not instructions for human use, and not a claim about any physiological effect. Nothing on this page is medical or dosing advice.
Research-use policy →The detail, in plain terms
Same 5mg, three concentrations.
The only thing that changes between these rows is how much bacteriostatic water you added. Each is reproduced as a laboratory reference for preparing a known concentration — not a human protocol.
- Vial contents
- 5mg lyophilized BPC-157 (pentadecapeptide), single vial.
- Add 1mL BAC water
- 5mg/mL = 5000mcg/mL → 50mcg per U-100 unit (most concentrated).
- Add 2mL BAC water
- 2.5mg/mL = 2500mcg/mL → 25mcg per unit (common, easy to measure).
- Add 3mL BAC water
- ~1.67mg/mL = ~1667mcg/mL → ~16.7mcg per unit (most dilute).
- Example draw
- 250mcg research aliquot at 2.5mg/mL = 0.1mL = 10 units.
- Diluent & storage
- Bacteriostatic water; add gently, no shaking; store reconstituted solution refrigerated.
Questions researchers ask
Before you order.
- How much bacteriostatic water do I add to a 5mg BPC-157 vial?
- There is no single required volume — the amount of water sets the concentration. Adding 1mL gives 5mg/mL, 2mL gives 2.5mg/mL, and 3mL gives roughly 1.67mg/mL. Researchers commonly choose 2mL because it produces a round 25mcg-per-unit figure on a U-100 syringe that is easy to measure. Pick the volume for the precision you need, then read the per-unit value off the concentration.
- How many units is a 250mcg research aliquot of BPC-157?
- It depends on how you reconstituted it. At 2.5mg/mL (a 5mg vial in 2mL), each U-100 unit carries 25mcg, so a 250mcg aliquot is 10 units (0.1mL). At 5mg/mL (1mL of water) it would be 5 units, and at ~1.67mg/mL (3mL) it would be 15 units. The concentration set during reconstitution is what converts a microgram figure into syringe units.
- Why does BPC-157 use such small syringe volumes?
- Because it is a microgram-scale compound. Research figures for BPC-157 are typically in the hundreds of micrograms, while a 5mg vial holds 5000mcg — so a single research aliquot is a small fraction of the vial and a small number of units on the syringe. That is the opposite of a milligram-scale compound like TB-500, where draws are much larger.
- Should I shake the vial to dissolve BPC-157 faster?
- No. Peptides can be degraded by mechanical shearing, so the water is run slowly down the vial wall and the solution is left to dissolve or swirled gently — never shaken. BPC-157 generally goes into solution readily. Aggressive mixing risks the integrity of the molecule you just paid to verify.
- Is BPC-157 approved for human use?
- Titan Peptide Lab supplies BPC-157 strictly as a research-use-only reagent for in-vitro laboratory work — not for human or animal consumption, and not for diagnostic, therapeutic, or preventative use. The reconstitution math on this page is a laboratory preparation reference, not medical or dosing advice.