How much bacteriostatic water do I add to a 5mg TB-500 vial?

The volume sets the concentration, so there's no single required amount. Adding 1mL gives 5mg/mL, 2mL gives 2.5mg/mL, and 3mL gives roughly 1.67mg/mL. A common choice is 2mL, because at 2.5mg/mL a 2.5mg research aliquot is a clean 1mL draw. Size the water so your planned aliquot lands on an easy syringe mark.

Why are TB-500 draws so much larger than BPC-157?

Because TB-500 is a milligram-scale compound while BPC-157 is microgram-scale. A research aliquot of TB-500 is measured in milligrams, so even at the same 2.5mg/mL concentration the draw is tens of units of a millilitre, not the handful of units BPC-157 uses. Same vial size, same dilution math, very different syringe volumes.

How many research aliquots does a 5mg TB-500 vial give?

Far fewer than a microgram compound — because TB-500 figures are milligram-sized, a single 5mg vial typically maps to just a couple of research aliquots. That's why the reconstitution volume is usually chosen so one planned aliquot is a convenient draw, rather than to stretch the vial across many uses.

Should I shake the vial to dissolve TB-500?

No. As with any peptide, the water is run slowly down the vial wall and the solution is swirled gently or left to dissolve — never shaken, because mechanical shearing can degrade the peptide. TB-500 generally goes into solution cleanly without aggressive mixing.

Is TB-500 approved for human use?

Titan Peptide Lab supplies TB-500 strictly as a research-use-only reagent for in-vitro laboratory work — not for human or animal consumption, and not for diagnostic, therapeutic, or preventative use. The reconstitution math on this page is a laboratory preparation reference, not medical or dosing advice.

TB-500 · 5mg lyophilized vial · reconstitution · research use only

TB-500 reconstitution: a milligram-scale compound, so the draws are large, not microscopic.

Titan's TB-500 ships as a 5mg lyophilized powder. The reconstitution arithmetic is the same dilution physics as any peptide — water volume sets concentration — but the scale is the part that trips people up. Research figures for TB-500 are quoted in milligrams, not micrograms, so a single 5mg vial is roughly one or two research aliquots rather than dozens, and the syringe draws are large fractions of a millilitre. This page works through the mg/mL math for 1mL, 2mL and 3mL, shows how a 5mg vial maps onto aliquots, and explains why the per-unit values look so different from a microgram compound like BPC-157. It is a laboratory reference, not a human protocol or dosing advice.

TB-500 5mg vial — $89.99 Open the reconstitution calculator

Same physics, milligram scale

TB-500 reconstitution follows the identical rule as every lyophilized peptide: the 5mg powder mass is fixed, and the bacteriostatic-water volume you add sets the concentration. What changes is the scale you operate at. Because TB-500 research figures are milligram-sized, you size the water volume around delivering whole or half milligrams per draw — not the tens of micrograms BPC-157 works in.

Which diluent to use →

The three common volumes, worked out

Add 1mL and 5mg dissolves into 5mg/mL — each U-100 unit carries 50mcg, and 1mg is 20 units. Add 2mL for 2.5mg/mL, where 1mg is 40 units (0.4mL) and a 2.5mg aliquot is a full 1mL draw. Add 3mL for ~1.67mg/mL. The spec table lays the three side by side so you can pick the volume that makes your target aliquot land on a clean syringe mark.

TB-500 research ranges →

One 5mg vial ≈ a couple of aliquots

Because TB-500 is dosed in milligrams in the literature, a 5mg vial is a small number of research aliquots rather than a long-running supply. That shapes the reconstitution choice: you generally pick a volume so a single planned aliquot is a convenient draw, knowing the vial will be used up quickly. It is the opposite logistics of a microgram compound where one vial lasts dozens of draws.

TB-500 half-life & cadence →

Add water gently, then store cold

Run the bacteriostatic water slowly down the vial wall rather than onto the powder, and let it dissolve or swirl gently — never shake. TB-500 generally reconstitutes cleanly. Once in solution it is handled as a refrigerated preparation with a finite working window, where the lyophilized cake was stable far longer; size the water volume around what you'll use before that window closes.

Storage & shelf-life notes →

Identity matters more for a 'TB-500' label

Accurate mg/mL math assumes the vial holds 5mg of the molecule you think it does — and 'TB-500' is a trade name that has been applied to more than one thing. A lot-matched COA should confirm the actual fragment by mass (the molecular weight tells you which molecule is present) and purity by HPLC, against the lot number on the vial. Concentration math is meaningless on a misidentified powder.

How to verify a COA →

Research-use framing

TB-500 is supplied strictly as a research-use-only reagent. The reconstitution arithmetic here describes how to prepare a known concentration for in-vitro and modelling work — it is dilution math, not instructions for human use, and not a claim about any physiological effect. Nothing on this page is medical or dosing advice.

Research-use policy →

The detail, in plain terms

5mg, sized for milligram draws.

The dilution rule is identical to any peptide; the difference is that TB-500's per-unit values land in milligram territory. Each row is reproduced as a laboratory reference for preparing a known concentration — not a human protocol.

Vial contents: 5mg lyophilized TB-500 (thymosin-β4-derived), single vial.
Add 1mL BAC water: 5mg/mL → 50mcg per U-100 unit; 1mg = 20 units (0.2mL).
Add 2mL BAC water: 2.5mg/mL → 25mcg per unit; 1mg = 40 units (0.4mL); 2.5mg = 1mL.
Add 3mL BAC water: ~1.67mg/mL → ~16.7mcg per unit; 1mg = 60 units (0.6mL).
Scale note: Milligram-scale compound — one 5mg vial is a small number of research aliquots.
Diluent & storage: Bacteriostatic water; add gently, no shaking; store reconstituted solution refrigerated.

Questions researchers ask

Before you order.

How much bacteriostatic water do I add to a 5mg TB-500 vial?: The volume sets the concentration, so there's no single required amount. Adding 1mL gives 5mg/mL, 2mL gives 2.5mg/mL, and 3mL gives roughly 1.67mg/mL. A common choice is 2mL, because at 2.5mg/mL a 2.5mg research aliquot is a clean 1mL draw. Size the water so your planned aliquot lands on an easy syringe mark.
Why are TB-500 draws so much larger than BPC-157?: Because TB-500 is a milligram-scale compound while BPC-157 is microgram-scale. A research aliquot of TB-500 is measured in milligrams, so even at the same 2.5mg/mL concentration the draw is tens of units of a millilitre, not the handful of units BPC-157 uses. Same vial size, same dilution math, very different syringe volumes.
How many research aliquots does a 5mg TB-500 vial give?: Far fewer than a microgram compound — because TB-500 figures are milligram-sized, a single 5mg vial typically maps to just a couple of research aliquots. That's why the reconstitution volume is usually chosen so one planned aliquot is a convenient draw, rather than to stretch the vial across many uses.
Should I shake the vial to dissolve TB-500?: No. As with any peptide, the water is run slowly down the vial wall and the solution is swirled gently or left to dissolve — never shaken, because mechanical shearing can degrade the peptide. TB-500 generally goes into solution cleanly without aggressive mixing.
Is TB-500 approved for human use?: Titan Peptide Lab supplies TB-500 strictly as a research-use-only reagent for in-vitro laboratory work — not for human or animal consumption, and not for diagnostic, therapeutic, or preventative use. The reconstitution math on this page is a laboratory preparation reference, not medical or dosing advice.